Research
Posters and Publications of the research I’ve done
ORCID: https://orcid.org/0000-0002-1872-7318
Yeo Lab (PhD) - Splicing mechanisms in translational medicine
I joined the Yeo Lab in October 2021. There are a lot of projects I’m working on, but my two major focuses are:
1) Collaborative project characterizing a nano-rare patient splicing mutation in sodium channel SCN8A. Our patient presents with pediatric epilepsy, and we’re developing antisense oligonucleotide treatments to treat the cause.
2) Developing a high-throughput screen that can be used to identify therapeutic regions-of-interest in splicing mutations/phenotypes. I’m looking at sodium channels for my thesis project, and determining the RNA-Binding-Proteins that regulate healthy and diseased splicing.
Check out some of my previous research accomplishments!
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Graduate Research Associate
Brian Zid Lab, UC San Diego
The Zid lab studies cell homeostasis and stress using biochemistry
Explored the effect of translational induction on mRNA localization during oxidative stress in mammalian cells
Created multiple biochemical assays and novel techniques to identify conserved sub-cellular behaviors between mammalian and yeast cells.
Developed method to stabilize fragile membrane-less compartments (p-bodies) for visualization by Fluorescence in-situ Hybridization.
Lead BSL2 mammalian cell culture division of lab, increasing project completion rate by 33%
Mentored two undergraduate students, one PhD candidate, and one Post-doctoral scholar in tissue culture experimental design/techniques.
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Graduate Research Associate
Roberto Malinow Lab, UC San Diego
The Malinow lab studies the neural basis for depression in rats and humansHypothesized and found evidence of a dual-regulatory circuit between the Lateral Habenula and Lateral Preoptic Area
Characterized the habenular-circadian circuit as putatively having long-range GABA-ergic and Glutamatergic projections. Identified galanin expressing projections from the LPO to the LHb
Evaluated the behavioral effects of sub-anesthetic doses of ketamine on a conditional learned helplessness rodent model
Identified discrete populations of galanin and ketamine induced neuronal activity using Fluorescence in-situ Hybridization and Immunohistochemistry
Performed major and minor surgical operations on rodents (i.e. cranial injections, parafomaldehyde perfusions)
Presented work at Lewis Judd Young Investigators Symposium
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Collaborated with Dr. Davidenko to continue face perception research from Bridgeman lab for publication
Designed, implemented, and analyzed experiments using Matlab software
Determined cognitive conflict between upper-visual-hemisphere bias and left gaze bias in facial perception
Wrote and revised manuscript for gaze bias Perception publication
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Laboratory Manager, Undergraduate Research Assistant
Bruce Bridgeman Lab, UC Santa Cruz
The Bridgeman lab studied the psychophysics visual cognition
Designed, developed, and implemented multi-modal experiments using human eye-tracking
Created analysis informatics scripts using Python and Matlab
Instituted formal laboratory procedure for data recording across multiple researchers
Determined spatial memory of angled distances using closed-loop walking
Identified left-gaze bias through a facial perception study
Determined cognitive load using cross-modal attentional bias experiments.
Submitted closed-loop research for publication
Closed lab after the passing of Dr. Bridgeman
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Undergraduate Research Assistant
Carrie Partch Lab, UC Santa Cruz
The Partch Lab studies the molecular clock using biochemistry
Identified Cancer-Testis antigen PASD1 as a circadian repressor
Developed circadian reporter lines from cancer cells
Developed novel circadian reporter line in suspension cells
Created siRNA knock-out systems using lentiviral mechanisms
Trained and instructed one graduate rotation student and two undergraduate researchers
Utilized persuasive writing and presentation skills in meetings and conferences
Developed grant proposal writing skills
Managed multiple research projects, coursework, and extracurricular activities simultaneously
Mastered mammalian cell culture protocol and techniques
Developed independent hypothesis of ALK5 receptor function and prospectus
Zid Lab — Stress GRanules
Glyoxal as an effective fixative for membrane-less granules in mammalian cells
Malinow Lab — Ketamine & Lateral Habenula
Posited the existence of a dual-regulation circuit mediating LHb activity through circadian neuropeptide regulation and acute neurotransmitter modulation in a depressed rodent model
Partch lab — PASD1
Developed a novel circadian reporter line. Characterized the cancer-testis antigen PASD1 as having peripheral circadian activity
Bridgeman Lab — Left Gaze Bias
Exploring the cognitive basis for the lateral gaze bias in human subjects
Bridgeman Lab — Slopes
Provided evidence discouraging “effort hypothesis” of slope perception
Bridgeman B, Kopalle H. Open-loop locomotion on slopes. Perception; under review March 2016
Davidenko Lab — Face Perception
Identifying the Left and Upper gaze biases of face perception